Inquiry number

INS-0000000373

ABSTRACT

 The Pharmaceutical Industry Beamline was constructed by the Pharmaceutical Consortium for Protein Structure Analysis (PCProt) which is composed of 22 pharmaceutical companies affiliating with the Japan Pharmaceutical Manufacturers Association (JPMA).
 The specification and equipments of this beamline is almost same as that of RIKEN Structural Genomics Beamline I & II.

AREA OF RESEARCH

• Protein structure analysis for structure-based drug design
  It means design and optimization of new leading compounds based on pharmacodynamic action mechanism elucidated at the molecular level which obtained from a detailed interaction analysis of receptor-drug complexes, etc.

KEYWORDS

• Scientific field
  Protein crystallography, Structure-based drug design, Drug discovery
• Equipment
  Diffractometer, Imaging plate detector, CCD detector

SOURCE AND OPTICS

 This Beamline has the SPring-8 standard transport channel and optics for bending magnet. Monochromatic X-rays from 7 to 17 keV can be obtained using standard double crystal monochromator and Rh-coated downward-deflection mirror with a typical glancing angle of 3.7 mrad. The X-ray Beam is focused on an sample using the bent cylindrical mirror.

• X-rays at sample

  Tunable energy range	7 ∼ 17.5 keV
  Energy resolution	ΔE/E ∼ 10-4
  Photon flux	∼ 1010 (12 keV) photon/s
  Beam size	0.2 mm (V) × 0.2 mm (H)

Schematic View of Beamline

EXPERIMENTAL STATIONS

  The total system of monochromatic data collection for routine protein crystallography and MAD (Multi-wavelength Anomalous Diffraction) experiments has been installed.

• Measurement
  • 2 quadrant slits
  • Gas-flow type ionization chamber
  • Imaging Plate detector for structural biology (Rigaku R-AXIS V) :
    Total active area of 400 mm × 400 mm and 100 µm pixels.
  • CCD detector for protein crystallography (Rigaku/MSC Jupiter210) :
    2 × 2 array of detector modules, a total active area of 210 mm × 210 mm and 51 µm pixels.
  • Si PIN photodiode detector and multi-channel analyzer for fluorescence measurement
• Sample
  • Sample stage
  • Horizontal kappa-type goniometer
  • Cryostream cooler (Nitrogen type) : temperature control range 80 ∼ 350 K
  • Coaxial microscope
• Others
  • Stereo microscope
  • Cryogenic tank

PUBLICATION SEARCH

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CONTACT INFORMATION

Please note that each e-mail address is followed by "@spring8.or.jp."

Kenji SUZUKI
Pharmaceutical Consortium for Protein Structure Analysis
1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148
Phone : +81-(0)791-58-1882
Fax : +81-(0)791-58-1883
e-mail : knjszk