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Fourth International Workshop on X-ray Damage to Biological Crystalline Samples
| Tuesday March 7th 2006 |
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Chair: Elspeth Garman (Oxford University) |
| 9:30 - 9:40 |
Welcome ……………………… Masaki Yamamoto + Soichi Wakatsuki |
| 9:40 - 9:50 |
Introduction …………………… Elspeth Garman |
| Session A: Relevant radiation chemistry. |
| 9:50 - 10:20 |
Ian Carmichael, Notre Dame Radiation Laboratory, Indiana, U.S.A.(abstract.1)
Radiation chemistry of aqueous amino acids. |
| 10:20 - 10:40 |
Andrea Schmidt, EMBL, Hamburg, Germany. (abstract.2)
How to avoid premature decay of your macromolecular crystal – a chemist's approach to combat physical reality.
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10:40 - 11:00
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Break
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Chair: Masaki Yamamoto (SPring-8) |
| Session B: Experiences from electron microscopy. |
| 11:00 - 11:30 |
William Massover, New Jersey Medical School, U.S.A. (abstract.3)
Radiation damage to protein specimens from modern electron microscope imaging and diffraction: a Minireview. |
| 11:30 - 12:00 |
Yoshinori Fujiyoshi, Kyoto University, Japan (abstract.4)
Cryo-protection and cryo-electron microscopy |
| 12:00 - 12:30 |
Tetsuya Ishikawa, RIKEN SPring-8 Center, Japan. (abstract.5)
SPring-8 Compact SASE Source (SCSS) |
12:30 - 13:40
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Lunch
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Chair: Raimond Ravelli (EMBL, Grenoble) |
| Session C: Dose/dose rate effects and limits to sample life. |
| 13:40 - 14:10 |
Gleb Bourenkov, MPI, Hamburg, Germany. (abstract.6)
Modeling the intensity variation with the X-ray dose. |
| 14:10 - 14:40 |
Nobutaka Shimizu, SPring-8, Japan (abstract.7)
Radiation damage of protein crystals at various X-ray energies |
| 14:40 - 15:10 |
Colin Nave, SRS, U.K. (abstract.8)
What is the optimum wavelength to study a protein crystal of a given size? |
| 15:10 - 15:40 |
Armin Wagner, DLS, Didcot, U.K.
Systematic study on the effect of cryocooling of protein crystals to 10K. |
15:40 - 16:00
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Break
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| 16:00 - 16:30 |
Elspeth Garman, University of Oxford, U.K. (abstract.10)
Experimental determination of the radiation dose limit for cryo-cooled protein crystals.
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Chair: Soichi Wakatsuki (KEK) |
| Session D: Imaging and Radiation Biology of the whole cell. |
| 16:30 - 17:00 |
Sean McSweeney, ESRF, France. (abstract.11)
Exploring an ionising radiation resistant phenotype. |
| 17:00 - 17:30 |
Yoshinori Nishino, RIKEN SPring-8 Center, Japan. (abstract.12)
High-spatial-resolution X-ray diffraction microscopy. |
| 17:30 - 18:00 |
Chris Jacobsen, Stony Brook University, U.S.A. (abstract.13)
Radiation damage at different scales: from molecules to cells. |
18:00 -19:40
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Evening Banquet sponsored by Rigaku.
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| Wednesday March 8th 2006 |
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Chair: Sean McSweeney (ESRF) |
| Session E: Improved software for dealing with radiation damage. |
| 8:30 - 9:00 |
Sasha Popov, EMBL, Hamburg, Germany. (abstract.14)
Handling the radiation damage in BEST. |
| 9:00 - 9:30 |
Marc Schiltz, Lausanne University, Switzerland. (abstract.15)
Detection and modeling of site-specific radiation damage in SAD/MAD experiments. |
| 9:30 - 10:00 |
Gerard Bricogne, Global Phasing, Cambridge, U.K. (abstract.16)
A statistical analysis of radiation damage effects suitable for a reformulation of scaling, merging, phasing and refinement. |
| 10:00 - 10:30 |
Zbyszek Otwinowski, UT South Western Medical Center Dallas, U.S.A. (abstract.17)
Complex impact of radiation damage on phasing. |
10:30 - 11:00
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Break
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Chair: Gerd Rosenbaum (University of Georgia) |
| Session F: Specific sensitivity of particular crystals/heavy atoms to damage. |
| 11:00 - 11:30 |
Raimond Ravelli, EMBL, Grenoble, France. (abstract.18)
Specific radiation damage versus global non-isomorphism. |
| 11:30 - 12:00 |
James Holton, ALS, Berkeley, U.S.A. (abstract.19)
A fluorescence probe for site specific damage. |
| 12:00 - 12:30 |
Udupi Ramagopal, AECOM, Bronx, U.S.A. (abstract.20)
Radiation-induced site-specific damage of mercury derivatives: phasing and implications. |
12:30 - 14:00
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Lunch
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Chair: Colin Nave (SRS) |
| Session G: The utilisation of radiation damage for biological studies. |
| 14:00 - 14:30 |
Tsutomu Kouyama, Nagoya University, Japan. (abstract.21)
Effects of X-ray radiation damage on crystallographic analyses of bacteriorhodopsin’s photoreaction intermediates. |
| 14:30 - 15:00 |
Kristina Djinovic-Carugo, University of Vienna, Austria. (abstract.22)
Reduction of metal centres by X-rays: case studies. |
| 15:00 - 15:30 |
Martin Weik, IBS, Grenoble, France. (abstract.23)
Specific radiation damage to acidic residues in protein crystals. |
| 15:30 - 16:00 |
Remco Kort, University of Amsterdam, Holland. (abstract.24)
Biological implications of radiation damage in the active site: cryocrystallographic studies on DNA photolyase and photoactive yellow protein. |
16:00 - 16:30
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Break
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| 16:30 - 17:00 |
Ilme Schlichting, MPI, Heidelberg, Germany. (abstract.25)
X-ray crystallographic studies of redox-sensitive proteins.
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Chair: Colin Nave (SRS) |
| Session H: Beam heating; modelling and verification. |
| 17:00 - 17:30 |
Eddie Snell, Hauptmann Woodward Institute, Buffalo, U.S.A. (abstract.26)
Thermal imaging applied to cryocrystallography: cryocooling and beam heating (Pt I). |
| 17:30 - 18:00 |
Michael Kazmierczak, University of Cincinnati, U.S.A (abstract.27)
Thermal imaging applied to cryocrystallography: beam heating of samples: modeling and verification (Pt II).
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| 18:00 - 18:30 |
Summary, discussion and close of Workshop.
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| Poster Presentation |
01. John McGeehan, EMBL, Grenoble, France.
Online Monitoring of UV/Vis Spectral Changes in Cryocooled Crystals (poster abstract.1)
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| On Monday afternoon 6th March at 16:00 and on Thursday morning 9th March (at 9:30am or 10:00am), there will be optional tours of SPring-8 lasting approximately 1.5 hours. |
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