問い合わせ番号

INS-0000000572

Protein Crystallography

  Rapid data collection system for protein crystallography is installed at BL38B1. Two detectors, CCD (ADSC/ Quantum315) and CMOS (Hamamatsu/ C10158DK), are equipped on the experimental table and users can easily change these detectors depending on their experimental purpose (Fig. 1). Combination of the coaxial-microscope to observe crystal along x-ray beam path and the goniometer having motor driven x, y and z stage make centering of crystal very easy (Fig. 2). Data collection, including measurement of XAFS spectrum and automatic MAD (Multi-wavelength Anomalous Diffraction) data collection, is performed through controlling software BSS (Beamline Scheduling Software). Since BSS can control all of the instruments required for data collection, switch of detector and change of camera length, wavelength are automatically performed at the beginning of measurement. Therefore, what beamline users have to do after crystal mounting and centering is to input measurement conditions to BSS.

Fig. 1. Diffractometer for protein crystallography
(a : ADSC/ Quantum315, b : Hamamatsu/ C10158DK)

 When CMOS is used, the CCD stage moves back and the CMOS stage can go up to the experimental table without any physical interference.

Fig.2. Magnified view around the goniometer
Two microscopes are used for crystal centering.
(a) low magnification microscope
(b) high magnification coaxial-microscope

• X-rays at sample

  Energy range	6 ∼ 17.5 keV
  Energy resolution	ΔE/E = 10-4
  Spot size	horizontally 90 µm, vertically 180 µm (@ 12.4 keV (λ = 1.0 Å)) at detector position

• Facilities
  • CCD detector (ADSC/ Quantum315)
    • Detector area : 315 × 315 mm²
    • Pixel size : 51 × 51 µm²
    • No. of pixels : 6144 × 6144
    • Camera length : 75 ∼ 950 mm
  • CMOS detector (Hamamatsu/ C10158DK)
    • Detector area : 117.6 × 117.6 mm²
    • Pixel size : 50 × 50 µm²
    • No. of pixels : 2352 ×2352
    • Camera length : 75 ∼ 330 mm
  • Horizontal axis goniometer having motor-driven x, y, z stage
  • Cryostat : temperature control range 80 ∼ 350 K
  • Gas-flow type ionization chamber
  • Si PIN photodiode detector for fluorescence measurement
  • Multi-channel analyzer of fluorescence measurement