Microtomographic observation of tumor blood vessels
問い合わせ番号
SOL-0000001182
ビームライン
BL20B2（医学・イメージングI）
学術利用キーワード
| A. 試料 | 生物・医学 |
|---|---|
| B. 試料詳細 | 生体組織、細胞系等 |
| C. 手法 | 吸収、及びその二次過程 |
| D. 手法の詳細 | |
| E. 付加的測定条件 | 三次元画像計測（ＣＴ等） |
| F. エネルギー領域 | Ｘ線（4～40 keV) |
| G. 目的・欲しい情報 | 形態・巨視的構造 |
産業利用キーワード
| 階層1 | 製薬, その他 |
|---|---|
| 階層2 | ドラッグデザイン |
| 階層3 | 薬物, 生体 |
| 階層4 | 形態 |
| 階層5 | イメージング |
分類
A80.90 その他, M60.20 Ｘ線ＣＴ
利用事例本文
A rabbit auricle specimen implanted with a carcinoma was fixed in formalin and sealed up in the specimen case after a barium sulphate solution was injected into blood vessels as a contrast agent. Projection images were recorded from 1800 views during 180-degree rotation. 3D images of the specimen shown in Fig. 1 were obtained by tomographic reconstruction, and small blood vessels induced by the tumor were demonstrated. An avascular area in the central portion was dead tumor tissue. In contrast, the number of blood vessels increased remarkably around the avascular area, where tumor cells were vital and a tumor's growth rate was rapid.
Fig. 1. 3D images of the tumor encircled with the network of blood vessels induced by the tumor.
The images are displayed at viewing angles of 0 (a front view), 30, and 60 degrees, from left to right.
画像ファイルの出典
所内報
誌名
SPring-8 Research Frontiers 2004
ページ
測定手法
A micro-computed tomography (micro-CT) system was developed using a 10-megapixel CCD camera for 3D biomedical imaging. The detector consists of a beryllium window for X-ray incidence, a fluorescent screen, an optical mirror, high numerical aperture lenses and the CCD camera. X-rays are converted into a visible image in the screen with thickness of 10 µm. The screen and the mirror are built in behind the beryllium window. The mirror deflects the luminescent light 90° to the lens system that focuses it on the camera. An X-ray field of view is 24mm wide by 16 mm high (4024 x 2648 pixels); the pixel measures 6 µm by 6 µm.
画像ファイルの出典
図なし
測定準備に必要なおおよその時間
3 時間
測定装置
| 装置名 | 目的 | 性能 |
|---|---|---|
| Micro-CT system | 3D-CT imaging | 10M pixels |
参考文献
| 文献名 |
|---|
| SPring-8 Research Frontiers 2004 |
関連する手法
No
アンケート
本ビームラインの主力装置を使っている
測定の難易度
中程度
データ解析の難易度
中程度
図に示した全てのデータを取るのにかかったシフト数
2～3シフト