SPring-8, the large synchrotron radiation facility

Inquiry number

SOL-0000001483

Beamline

BL23SU (JAEA Actinide Science II)

Scientific keywords

A. Sample category	organic material, atom, molecule, radical, biology, medicine
B. Sample category (detail)	macromolecule, neutral molecule, biology (in vitro), organism, cell, biological material, nucleic acid
C. Technique	absorption and its secondary process
D. Technique (detail)	XANES
E. Particular condition	polarization (circular), ultra-high vacuum, room temperature
F. Photon energy	soft X-ray
G. Target information	chemical state

Industrial keywords

level 1---Application area	environment, Pharmaceuticals
level 2---Target	drug design, food
level 3---Target (detail)	protein, drug
level 4---Obtainable information	film thickness, orientation (preferred orientation), chemical state
level 5---Technique	NEXAFS

Classification

A40.40 surface・interface chemistry, M40.40 soft x-ray spectroscopy

Body text

固体薄膜に対するXANES強度の入射光と薄膜表面との角度依存性の測定は基板上の分子の配向角度を調べることのできる簡便な手法です。この手法を用いることで、薄膜上の異なる元素を選択して配向角度を測定することができます。BL23SUの単色軟X線を利用して、窒素や酸素などの軽元素の内殻電子を選択して励起することが可能になります。図に示すのは、DNA構成塩基（アデニン、グアニン、ウラシル）について測定した窒素K殻領域のXANESの*強度の入射光と基板との角度変化です。この結果から、アデニン、グアニンおよびウラシルはそれぞれ基板に対して15、38および16傾いて配向していることがわかりました。一方、シトシンおよびチミンはランダムな配向を持つことがわかりました。

Fig. 1 Polarization dependence of the intensities of the * resonances in the N 1s NEXAFS spectra of nucleobases. The symbols (●■▲) show the normalized peak area ratios of the lowest * resonance of adenine, guanine, and uracil obtained from the curve-fitting procedure. Solid, dashed, and dotted lines show the curve-fitting results for adenine, guanine, and uracil, respectively.

[ K. Fujii, K. Akamatsu and A. Yokoya, The Journal of Physical Chemistry B 108, 8031-8035 (2004), Fig. 4,
©2004 American Chemical Society ]

Source of the figure

Original paper/Journal article

Journal title

The Journal of Physical Chemistry B 108 (2004) 8031-8035

Figure No.

4

Technique

角度分解XANES分光法は、放射光の入射光と表面との角度を変えながらXANES測定を行うことによって得られます。この例では、DNA塩基の基板に対する配向角度がえられます。

Fig. 2 Illustration of the measurement chamber for ion desorption.
The vacuum chamber is connected to the beam-line vacuum pipe.

[ K. Fujii, K. Akamatsu and A. Yokoya, Radiation Research 161, 435-441 (2004), Fig. 1,
©2004 Radiation Research Society ]

Source of the figure

Original paper/Journal article

Journal title

K. Fujii et. al., Radiation Research 161 (2004) 435-441

Figure No.

1

Required time for experimental setup

1 hour(s)

Instruments

Instrument	Purpose	Performance
生物物理学的分光装置	光脱離イオン観測	1~510amu

References

Document name
K. Fujii et. al., The Journal of Physical Chemistry B 108 (2004) 8031-8035
K. Fujii et. al., Nuclear Instruments and Methods in Physics Research B 199 (2003) 249-254
藤井健太郎、放射光　16 (2003) 17-24

Related experimental techniques

Questionnaire

This solution is an application of a main instrument of the beamline.

Ease of measurement

Easy

Ease of analysis

Middle

How many shifts were needed for taking whole data in the figure?

Two-three shifts