SPring-8, the large synchrotron radiation facility

Inquiry number

INS-0000001513

ABSTRACT

  Most of proteins substantially important in biological research have difficulties in growing single crystals of sufficient size. The beamline BL32XU is dedicated to 'protein micro-crystallography' by using micro-sized crystals. Stable brilliant micro-beam with size ranging 1 µm to 15 µm, a rapid-readout detector (EIGER X 9M), a high precision air-bearing goniometer, an automated sample changer SPACE are available. Recently, a fully automated data collection system, ZOO^{[#1, #2]}, has been developed at this beamline. The system enables all of possible 'goniometer-based' data collection schemes such as 'normal(rotation crystallography)', ‘multiple small wedge', 'helical', 'serial synchrotron rotation' schemes. Additionally, the beamline has much experienced in experimental phasing and high resolution structure analyses of membrane protein crystals (Please refer the publication list).
  Available X-ray energy ranges 9-15 keV. Available beamsize ranges 1×1 µm ~ 10(H)×15(V) µm with roughly same photon flux density of 7×10¹⁰ photons/sec/µm² in all beam sizes.

[#1] K. Hirata, K. Yamashita, et al. “ZOO: an automatic data-collection system for high-throughput structure analysis in protein microcrystallography”, Acta Cryst (2019). D75, no. 2, 138–150.
[#2] K. Yamashita, K. Hirata, and M. Yamamoto, “KAMO: towards automated data processing for microcrystals”, Acta Cryst (2018). D74, no. 5, 441–449.

AREA OF RESEARCH

• Structural biology
• X-ray crystallography
• Micro crystallography

Keywords

• Specification
  - Micro focus
  - High flux density
• Equipments
  - Focusing mirrors fabricated with EEM technique
  - Hybrid photon counting detector

SOURCE AND OPTICS

  The beamline consists of a hybrid in-vacuum undulator, a liquid-nitrogen cooled double crystal monochromator, and K-B focusing mirrors with large reduction ratio. High precision monochromator has been specially designed based on the SPring-8 standard double crystal monochromator. The focusing mirror surfaces are fabricated in atomic level accuracy with Elastic Emission Machinery (EEM) technique. The beam size is variable with a high-precision slit as virtual light source according to designed experiments.

EXPERIMENTAL STATIONS

The ultra-high precision diffractometer is being developed now.

PUBLICATION SEARCH

* Sorry, Some parts of results are displayed using Japanese characters.

BL32XU PUBLICATION SEARCH

CONTACT INFORMATION

Please note that each e-mail address is followed by "@riken.jp"

Kunio Hirata
RIKEN/SPring-8 center
Kouto 1-1-1, Sayo-cho, Sayo-gun, Hyogo,
679-5148 JAPAN　
Phone : +81-(0)791-58-2839
Fax : +81-(0)791-58-2834
e-mail : kunio.hirata