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BL38B1 OUTLINE

Inquiry number

INS-0000000577

ABSTRACT

  RIKEN Structural Biology I (BL38B1) is a BioSAXS beamline for the purpose of structure analysis of proteins, nucleic acids and these complex in a solution. BioSAXS of BL38B1 has been relocated from BL45XU in 2019.

AREA OF RESEARCH

  • Structure biology based on SAXS and WAXS/D

KEYWORDS

  • Scientific field
    Structure biology, BioSAXS, SAXS, WAXS/D
  • Equipment
    Photon Counting Area Detector (PILATUS3X 2M), Solution cell with controllable temperature (4 ∼ 60℃), Online SEC-SAXS (SHIMADZU HPLC)

SOURCE AND OPTICS

  The optics of the beamline consists of standard bending magnet components. A unsymmetrical Si(111) double crystal monochromator followed by a Rh courted bend cylindrical focusing mirror is installed in the optics station. The focusing point is fixed at the area detector surface in the experimental station. The optical specification is shown in the following table.

  • Optical specification of BL38B1

    Energy range About 6.5 ∼ 14 keV
    (Available energy on user operation is either 12.4 keV to 10.0 keV.)
    Energy resolution ΔE/E = 10-4
    Photon flux @ sample position ∼ 1010 photons/sec/100 mA
    Typical beam size @ sample position with camera length of 2.5 m 700 µm(h) × 150 µm(v)(FEHM)

    Schematic View of Beamline

    EXPERIMENTAL STATIONS

    The experimental station equips PILATUS3X 2M at the position that is fixed with length of about 52 m from X-ray source and an experimental stage. Monochromatic X-ray is shaped by a scatterless slits at the front in the station and a parasitic scattering is removed by another scatterless slits at the front of a sample. In user operation, the sample to camera length is fixed either 2.5 m or 0.3 m. Measurable q range is shown in the following table.

    • Measurable q range with each camera length and wave length

      Camera length (m) Wave length(Å)* dmax(Å) dmin(Å) qmin(Å-1)** qmax(Å-1)**
      2.5 m 0.9 1652 23.3 0.0038 0.27
      1.0 1460 21.0 0.0043 0.30
      1.2 1250 17.5 0.0050 0.36
      1.5 995 14.0 0.0063 0.45
      0.3 m 1.0 89 1.7 0.07 3.8

      * Wave length at user operation is either 1.0 Å or 1.2 Å.
      ** q = 2π/ d

    Experimental stage in the experiment station

    PUBLICATION SEARCH

    * Sorry, Some parts of results are displayed using Japanese characters.

    BL38B1 PUBLICATION SEARCH

    CONTACT INFORMATION

    Please note that each e-mail address is followed by "@riken.jp".

    Takaaki HIKIMA
    SR Life Science Instrumentation Team, Life Science Research Group,
    Advanced Photon Technology Division, RIKEN SPring-8 Center
    1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5198
    e-mail : takaaki.hikima

Last modified 2021-04-16 15:28
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