SPring-8, the large synchrotron radiation facility

Inquiry number

INS-0000000577

ABSTRACT

  RIKEN Structural Biology I (BL38B1) is a BioSAXS beamline for the purpose of structure analysis of proteins, nucleic acids and these complex in a solution. BioSAXS of BL38B1 has been relocated from BL45XU in 2019.

AREA OF RESEARCH

• Structure biology based on SAXS and WAXS/D

KEYWORDS

• Scientific field
  Structure biology, BioSAXS, SAXS, WAXS/D
• Equipment
  Photon Counting Area Detector (PILATUS3X 2M), Online SEC-SAXS (SHIMADZU HPLC)

SOURCE AND OPTICS

  The optics of the beamline consists of standard bending magnet components. A unsymmetrical Si(111) double crystal monochromator followed by a Rh courted bend cylindrical focusing mirror is installed in the optics station. The focusing point is fixed at the area detector surface in the experimental station. The optical specification is shown in the following table.

• Optical specification of BL38B1

  Energy range	About 6.5 ∼ 14 keV (Available energy on user operation is 12.4 keV.)
  Energy resolution	ΔE/E = 10-4
  Photon flux @ sample position	∼ 1011 photons/sec/100 mA
  Typical beam size @ sample position with camera length of 2.5 m	400 µm（h） × 300 µm（v）（FWHM）

  

  Schematic View of Beamline

  EXPERIMENTAL STATIONS

  The experimental station equips PILATUS3X 2M at the position that is fixed with length of about 52 m from X-ray source and an experimental stage. Monochromatic X-ray is shaped by a scatterless slits at the front in the station and a parasitic scattering is removed by another scatterless slits at the front of a sample. In user operation, the sample to camera length is fixed either 2.5 m or 0.3 m. Measurable q range is shown in the following table.

  • Measurable q range with each camera length and wave length

    Camera length (m)	Wave length（Å）*	dmax（Å）	dmin（Å）	qmin（Å-1）**	qmax（Å-1）**
    2.5 m	1.0	1047	14.6	0.006	0.43
    0.3 m	1.0	150	1.9	0.04	3.3

    * Wave length at user operation is 1.0 Å.
    ** q = 2π/ d

  

  Experimental stage in the experiment station

  PUBLICATION SEARCH

  * Sorry, Some parts of results are displayed using Japanese characters.
  BL38B1 PUBLICATION SEARCH

  CONTACT INFORMATION

  Please note that each e-mail address is followed by "@spring8.or.jp".
  

  Hiroshi SEKIGUCHI
  SPring-8 / JASRI
  1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5198
  Phone : +81-(0)791-58-0833
  Fax : +81-(0)791-58-2512
  e-mail : sekiguchi

  Hiroyasu MASUNAGA
  SPring-8 / JASRI
  1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5198
  Phone : +81-(0)791-58-0833
  Fax : +81-(0)791-58-2512
  e-mail : masunaga