Inquiry number

INS-0000000343

Protein crystal diffractometer

  The diffractometer is designed for protein X-ray diffraction experiment. This diffractometer consists of three parts: X-ray beam shaper, goniometer and detector base.
  The X-ray beam shaper has two sets of quadrant slits, two rotary attenuators, two beam intensity monitors, collimator and shutter. The size, shape and intensity of X-ray beam at sample position is changeable by using these components.
  The high-speed air-bearing goniometer has a sample rotation axis (ω). Three translation axes (X, Y and Z) are equipped in this goniometer for semi-automatic sample centering. Two types of cryostream coolers, nitrogen gas and helium gas, are installed on the diffractometer. These cryostream coolers are open-flow type, and the cryostream cooler using nitrogen gas can cool a sample to ∼ 90 K and the helium gas can do ∼ 35 K.
  Two detectors (Rayonix MX225HE X-ray CCD detector and RIGAKU R-AXIS V imaging plate detector) are installed at BL41XU. However, MX225HE is always set up on the detector base so that most users may use CCD. If you want to use the IP detector, it is necessary to describe that to the application form when you submit the proposal.
  Robotic Sample Handling Systems, SPACE developed at SPring-8 is also installed and available. Since this SPACE has been equipped with a newly developed attachment parts for usual metal magnet sample pins, users can select sample pin type, SPACE-specially screw pins or metal magnet pins.

• Specifications of X-ray beam shaper
  • Minimum beam size at sample position : 0.030 × 0.030 mm²
  • Beam intensity attenuator : Al 50 ∼ 6000 μm
• Specifications of goniometer
• Sample rotation axis (ω): not ristricted  
• Sample translation axis (X): ± 4.0 mm, (Y): ± 15.0 mm, (Z): ± 4.0mm
• Rotation speed : 180 ° / sec.
• Temperature of cryostream : 300 ∼ 90 K (nitrogen gas) ∼ 35 K (helium gas)
• Specifications of detector / Rayonix MX225HE
  • Detector area : 225 × 225 mm²
  • CCD size : 3072 × 3072 pixel
  • Pixel size : 0.073 × 0.073 mm²
  • ADC : 16 bit
  • Read-out time : 1.0 sec.
  • Total turn-over time : 2.3 sec. (without exposure time)
  • Camera length: 70 ~ 585 mm
• Specifications of detector / RIGAKU R - AXIS V
  • Detector area : 400 × 400 mm²
  • Pixel size : 0.1 × 0.1 mm²
  • Read-out time : 50 sec.
  • Total turn-over time : 1 min
  • Camera length : 155 ∼ 600 mm
• SPring-8 Precise Automatic Cryo-sample Exchanger (SPACE)
• Peltiert-cooled Si PIN photodiode
• Multi-channel analyzer
• Cryo-Xe-siter
• Cryo-stream-shutter for annealing
• Microscope
• Incubator (4, 20°C)
• Cold room (5°C)
• Liquid nitrogen dewar vessel