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BL44XU OUTLINE

Inquiry number

INS-0000000389

ABSTRACT

  This beamline is specially designed to collect high quality X-ray diffraction data of biological macromolecular assemblies, e.g. protein complexes, protein-nucleic acid complexes, and viruses.

AREA OF RESEARCH

  • Crystal structure analysis of biological macromolecular assemblies (e.g. membrane protein complexes, protein complexes, protein-nucleic acid complexes, and viruses)

KEYWORDS

  • Scientific field
    Biological macromolecular assemblies, Protein crystallography, Structural biology
  • Equipment
    High precision goniometer, Imaging plate detector, CCD detector, Cryostream cooler

SOURCE AND OPTICS

  Main beamline optics is a double-crystal monochromator and a horizontal focusing mirror.

Insertion device In-vacuum undulator
Undulator period : λu 32 mm
Number of period : Nperiod 140
Tunable range 9 ∼ 16 keV

Schematic View of Beamline

  • X-rays at sample

    Energy Range 7 ~ 17.5 keV
    Energy Resolution ΔE/E < 2 × 10-4
    Photon Flux 3 × 1011 photons/s
    Beam Size 0.05(H) × 0.05(V) mm2

EXPERIMENTAL STATIONS

  Two individual single rotation axis (horizontal and vertical) goniometers are available in the experimental hutch.

  • Goniometer

    Deviation from rotation axis ± 2.5 µm
    Position reproducibility ± 0.001 deg.
    Sample translation range ± 30 mm
    Sample-to-detector distance 250 ∼ 1000 mm
    Collimation Quadrant slits
    Sample camera CCD camera (× 250)

  • Cryostream cooler

    Temperature range 90 ∼ 300 K (N2 gas)
    35 ∼ 300 K (He gas)
    Nozzle inside diameter 7mmφ

  • Detectors   DIP-6040 (Bruker-AXS) can be used. DIP6040 has a CCD detector (Bruker-AXS SMART6500) surrounded by independent six imaging plate detector systems.
    • DIP6040
      • Imaging plate detector parts

        Number of imaging plates 6
        Size of an imaging plate 400 mmφ
        Readout system pulse motor control
        IP changer Spiral
        ADC 16 bit
        Size of pixel 100µm × 100µm
        Dynamic range >106
        Sensitivity < 1.0 photon/pixel
        Partial reading Available (< 400 mmφ)
        Readout time 400 mmφ : 120 frame/hr.

      • CCD part (Smart-6500)

        Sensitive area 165 mmφ
        Number of CCDs 1
        Demagnification of fiberoptic tapers 2.75 : 1
        Conversion gain (12 keV) 18 e-/x-ray photons
        Pixels 2048 × 2048
        Pixel size at input (avg.) 80 µm
        ADC 16 bit
        Data transfertime 10 sec.

        Main data processing, structure determination software
        • MOSFLM
        • HKL2000 (DIP6040, SMART6500)
        • CCP4

PUBLICATION SEARCH

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BL44XU PUBLICATION SEARCH

 

CONTACT INFORMATION

Atsushi NAKAGAWA
Research Center for Structural and Functional Proteomics
Institute for Protein Research, Osaka University
3-2 Yamadaoka, Suita, Osaka, 565-0871
Phone : +81-(0)6-6879-4313
Fax : +81-(0)6-6879-4313
e-mail : m1.png

Mamoru SUZUKI
Research Center for Structural and Functional Proteomics
Institute for Protein Research, Osaka University
3-2 Yamadaoka, Suita, Osaka, 565-0871
Phone : +81-(0)6-6879-8637
Fax : +81-(0)6-6879-4313
e-mail : m2.png

Eiki YAMASHITA
Research Center for Structural and Functional Proteomics
Institute for Protein Research, Osaka University
3-2 Yamadaoka, Suita, Osaka, 565-0871
Phone : +81-(0)6-6879-8637
Fax : +81-(0)6-6879-4313
@ SPring-8
Phone: 0791-58-1814
Fax : 0791-58-1814
e-mail : m3.png

Last modified 2013-06-04 11:43
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