Inquiry number

INS-0000000389

ABSTRACT

  This beamline is specially designed to collect high quality X-ray diffraction data of biological macromolecular assemblies, e.g. protein complexes, protein-nucleic acid complexes, and viruses.

AREA OF RESEARCH

• Crystal structure analysis of biological macromolecular assemblies (e.g. membrane protein complexes, protein complexes, protein-nucleic acid complexes, and viruses)

KEYWORDS

• Scientific field
  Biological macromolecular assemblies, Protein crystallography, Structural biology
• Equipment
  High precision goniometer, Imaging plate detector, CCD detector, Cryostream cooler

SOURCE AND OPTICS

  Main beamline optics is a double-crystal monochromator and a horizontal focusing mirror.

Schematic View of Beamline

• X-rays at sample

  Energy Range	7 ~ 17.5 keV
  Energy Resolution	ΔE/E < 2 × 10-4
  Photon Flux	3 × 1011 photons/s
  Beam Size	0.05(H) × 0.05(V) mm2

EXPERIMENTAL STATIONS

  Two individual single rotation axis (horizontal and vertical) goniometers are available in the experimental hutch.

• Goniometer

  Deviation from rotation axis	± 2.5 µm
  Position reproducibility	± 0.001 deg.
  Sample translation range	± 30 mm
  Sample-to-detector distance	250 ∼ 1000 mm
  Collimation	Quadrant slits
  Sample camera	CCD camera (× 250)

• Cryostream cooler

  Temperature range	90 ∼ 300 K (N2 gas) 35 ∼ 300 K (He gas)
  Nozzle inside diameter	7mmφ

• Detectors   DIP-6040 (Bruker-AXS) can be used. DIP6040 has a CCD detector (Bruker-AXS SMART6500) surrounded by independent six imaging plate detector systems.
  
  • DIP6040
    • Imaging plate detector parts

      Number of imaging plates	6
      Size of an imaging plate	400 mmφ
      Readout system	pulse motor control
      IP changer	Spiral
      ADC	16 bit
      Size of pixel	100µm × 100µm
      Dynamic range	>106
      Sensitivity	< 1.0 photon/pixel
      Partial reading	Available (< 400 mmφ)
      Readout time	400 mmφ : 120 frame/hr.

    • CCD part (Smart-6500)

      Sensitive area	165 mmφ
      Number of CCDs	1
      Demagnification of fiberoptic tapers	2.75 : 1
      Conversion gain (12 keV)	18 e-/x-ray photons
      Pixels	2048 × 2048
      Pixel size at input (avg.)	80 µm
      ADC	16 bit
      Data transfertime	10 sec.

      Main data processing, structure determination software
      • MOSFLM
      • HKL2000 (DIP6040, SMART6500)
      • CCP4

PUBLICATION SEARCH

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CONTACT INFORMATION

Atsushi NAKAGAWA
Research Center for Structural and Functional Proteomics
Institute for Protein Research, Osaka University
3-2 Yamadaoka, Suita, Osaka, 565-0871
Phone : +81-(0)6-6879-4313
Fax : +81-(0)6-6879-4313
e-mail :

Mamoru SUZUKI
Research Center for Structural and Functional Proteomics
Institute for Protein Research, Osaka University
3-2 Yamadaoka, Suita, Osaka, 565-0871
Phone : +81-(0)6-6879-8637
Fax : +81-(0)6-6879-4313
e-mail :

Eiki YAMASHITA
Research Center for Structural and Functional Proteomics
Institute for Protein Research, Osaka University
3-2 Yamadaoka, Suita, Osaka, 565-0871
Phone : +81-(0)6-6879-8637
Fax : +81-(0)6-6879-4313
@ SPring-8
Phone: 0791-58-1814
Fax : 0791-58-1814
e-mail :