Inquiry number

INS-0000000389

ABSTRACT

  This beamline is specially designed to collect high quality X-ray diffraction data of biological macromolecular assemblies, e.g. protein complexes, protein-nucleic acid complexes, and viruses.

AREA OF RESEARCH

• Crystal structure analysis of biological macromolecular assemblies (e.g. membrane protein complexes, protein complexes, protein-nucleic acid complexes, and viruses)

KEYWORDS

• Scientific field
  Biological macromolecular assemblies, Protein crystallography, Structural biology
• Equipment
  High precision goniometer, Imaging plate detector, CCD detector, Cryostream cooler

SOURCE AND OPTICS

  Main beamline optics is a double-crystal monochromator and a horizontal focusing mirror.

Schematic View of Beamline

• X-rays at sample

  Energy (wavelength) Range	6.5 ~17.7 keV (1.9 Å〜0.7 Å)
  Energy Resolution	ΔE/E < 2 × 10-4
  Photon Flux	3.0 × 1012 photons/s (φ50 µm)
  Beam Size	φ70 µm, φ50 µm, φ30 µm, φ20 µm, φ10 µm

EXPERIMENTAL STATION

• Goniometer

  Rotation axis range	-720 ~ 720 deg.
  µ axis	0 ~ 15 deg.
  Deviation from rotation axis	< ± 0.5 µm
  Rotation speed	1 sec / 180 ゜
  Collimation	Quadrant slits or pinholes
  Sample camera	CCD camera (× 250)

• Cryostream cooler

  Temperature range	90 ∼ 300 K (N2 gas) 35 ∼ 300 K (He gas)
  Nozzle inside diameter	7mmφ

• Sample changer
  SPACE-II
• Detector
  Eiger X 16M (Dectris)

  Number of detector modules	4 x 8
  Active area (width x height)	311.2 x 327.8 mm2
  Pixel size	75 x 75 µm2
  Inactive area	6.6 %
  PSF	1 pixel
  Maximum frame rate	133 Hz
  Maximum count rate	5 x 108 photons/s/mm2
  Counter bit depth	12 bit
  Image bit depth	16 or 32 bit
  Data format	HDF5 / NeXus

Sample to detector distance	115 – 1200 mm
Offset (vertical)	0 – 150 mm
2θ	0 – 15°

• Main data processing, structure determination software
  • XDS
  • MOSFLM
  • autoPROC
  • CCP4

PUBLICATION SEARCH

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BL44XU PUBLICATION SEARCH

CONTACT INFORMATION

Atsushi NAKAGAWA
Research Center for Structural and Functional Proteomics
Institute for Protein Research, Osaka University
3-2 Yamadaoka, Suita, Osaka, 565-0871
Phone : +81-(0)6-6879-4313
Fax : +81-(0)6-6879-4313
e-mail :


Eiki YAMASHITA
Research Center for Structural and Functional Proteomics
Institute for Protein Research, Osaka University
3-2 Yamadaoka, Suita, Osaka, 565-0871
Phone : +81-(0)6-6879-8635
Fax : +81-(0)6-6879-4313
@ SPring-8
Phone: 0791-58-1814
Fax : 0791-58-1814
e-mail :


Website

http://www.protein.osaka-u.ac.jp/rcsfp/supracryst/en/research/beamline/