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Chronobiologists identify key circadian clock mechanism in cyanobacteria(Press Release)

Release Date
06 May, 2022
  • BL44XU (Macromolecular Assemblies)

6 May, 2022

The activation and inactivation mechanisms of a key protein involved in the circadian clock system of cyanobacteria—an important organism in the evolution of such internal clocks—have long eluded scientists. But researchers have now identified how the system is driven.

(Information of the paper)
Journal Name: Proceedings of the National Academy of Sciences of the United States of America (PNAS)
Journal Title: Regulation Mechanisms of the Dual ATPase in KaiC
Authors: Yoshihiko Furuike, Atsushi Mukaiyama, Shinichi Koda, Damien Simon, Dongyan Ouyang, Kumiko Ito-Miwa, Shinji Saito, Eiki Yamashita, Taeko Nishiwaki-Ohkawa, Kazuki Terauchi, Takao Kondo, Shuji Akiyama
DOI: 10.1073/pnas.2119627119

Researchers have identified a key mechanism involved with the setting of the circadian clock of cyanobacteria—a model organism for study by chronobiologists due to the organism having one of the earliest circadian systems to evolve, and thus shining a light on how our own such systems work.

A paper describing the findings appeared in the Proceedings of the National Academy of Sciences on 4th May, 2022.

Chronobiologists—researchers who study the timing processes, including circadian clocks, of organisms—have long been interested in cyanobacteria (aka blue-green algae) as a model organism for investigation, and its KaiC protein in particular.

The KaiC protein forms a key part of the cyanobacteria’s master clock, and regulation of the genes that produce this protein and others it interacts with is crucial for maintaining the bacterium’s circadian rhythm, and thus when to engage in its core life processes such as photosynthesis and cell division. Further elucidation of how the system works thus shines a light on how circadian clocks work throughout the living world.

KaiC is an ATPase, an enzyme that initiates (catalyzes) the chemical reaction that splits off a phosphoryl group (an ion containing phosphorus and oxygen) from adenosine triphosphate (ATP) by using a water molecule, a process that releases energy that can then be harnessed to power actions throughout living things.

But KaiC is a special type of ATPase in that it has a double-domain structure, with one active site (location on an enzyme where the chemical reaction takes place) in one domain and another active site in the other. The cyanobacteria’s circadian clock system is governed through a slow and orderly—but also very complex—coordination of the two sites (Figure 1).

To do this, the KaiC protein uses two types of ATP molecules to produce diverse chemical reactions and thereby govern the circadian rhythm. The ATP molecules attach themselves to a Walker motif—a loop structure in proteins that is associated with phosphate binding—present in the two domains, called N-terminal C1 and C-terminal C2. The ATP molecule bound to the C1 domain is the main source of the ATP hydrolysis reaction whose rate determines the speed of the clock system. In the presence of KaiC’s sister proteins KaiA and KaiB, the ATP molecules bound not only in the C1 domain but also in the C2 domain are activated and then inactivated periodically (left upper panel of Figure 2).

“In recent years, this C1/C2-ATPase interaction of KaiC has become an important research target to achieve a better understanding of the circadian clock system in cyanobacteria,” said Shuji Akiyama, a biophysicist with the Institute for Molecular Science at Japan’s National Institutes of Natural Sciences, and co-author of the study, “as it closely relates to the clock’s properties of oscillation, period-tuning, and adjustment of the system to compensate for the effects of changes in temperature.”

A great deal of research has explored KaiC ATPase’s biochemistry and structure, but the precise mechanisms of its activation and inactivation until now have remained unknown.

The researchers used biochemical and structural biology techniques, including substitutions of amino acids in KaiC itself, to characterize the properties and interplay of the dual ATPase active sites. They also performed an analysis of the crystal structure of KaiC to visualize the activated and inactivated forms of the ATP and catalytic water molecules in the C1 domain (Right upper panels of Figure 2).

They found that the N-terminal and C-terminal ATPases communicate with each other through an interface between the N-terminal and C-terminal domains in KaiC. The dual ATPase sites are regulated rhythmically in a concerted or opposing manner depending on the phase of the circadian clock system, to control the assembly and disassembly cycle of the other clock proteins, KaiA and KaiB. The results suggest that the activation of dual KaiC ATPases through an auto-catalytic mechanism (a product of a reaction then becomes a catalyst for the same reaction, lower panel of Figure 2,) contributes to a sudden disassembly at dawn of the protein complexes built over night.

This is crucial for resetting “subjective night”, or what the organism’s clock predicts the length of night to be, and then pushing the whole system forward in its cycle.

Many structural details of the C2-ATPase remain unclear even after the researchers’ analysis, partly because they were unable to identify some catalytic water molecules involved, suggesting further areas of research to fine-tune their understanding of the clock system. The researchers were also amazed at the enormous range of activity of the C2-ATPase, which can be suppressed down to zero. The physiological significance of this is also the next important research target for the scientists.


Figure 1: Role of KaiC ATPase in cyanobacterial circadian clock
The clock can be reconstituted in a test tube in the presence of ATP (right upper panel). Dual ATPases in KaiC are activated and inactivated rhythmically (right lower panel) to provide a timing cue for the assembly and disassembly of KaiA and KaiB (left panel).


Figure 2: Activation and inactivation of dual ATPases drive the clock system
The ATPase activity is inactivated during the evening and night, but recovers autonomously at dawn and is further activated in the morning (left upper panel). The relative position of a catalytic water molecule determines the reaction efficiency (right upper panel). Protein complexes are autonomously disassembled by KaiA-assisted auto-catalytic activation of dual ATPases to terminate the subjective night (lower panel).

(Financial Support)
Grants-in-Aid for Scientific Research (17H06165, 18K06171, 19K16061)


(Contact Person)
Shuji Akiyama
TEL: +81-564-55-7363
E-mail: akiyamasatims.ac.jp

Yoshihiko Furuike
TEL: +81-564-55-7336
E-mail: furuikeatims.ac.jp

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