SPring-8, the large synchrotron radiation facility

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123rd SPring-8 Seminar

Subtitle/Subject TiO2-DNA Nanocomposites as Vehicles for Intracellular Manipulation
Period to Aug 27 , 2004
Venue Kamitsubo Hall
Host/Organizer JASRI/SPring-8
Format Lecture
Fields Materials Science, Life Science, Industrial Applications

Date&Time: 16:00 - 17:00, 27 August 2004

Speaker: Prof. Gayle E. Woloschak
Affiliation: Northwestern University, Feinberg School of Medicine, Department of Cell and Molecular Biology

  In order to develop TiO2-biopolymer nanocomposites as new vehicles for biotechnology-inducible gene specific endonucleases, we need to establish that oligonucleotides attached to TiO2 nanoparticle show sequence specificity with regard to targeting different cellular locations. Inside cells, DNA can be found both in the nucleus and in mitochondria. Previously, TiO2-nanocomposites targeted for the nucleus had been demonstrated [1, 2]. We used nanocomposites with oligonucleotides matching the mitochondrial genome of the rat [5' carboxy dTccactttccacacag (MIT1s-TiO2) present in mitochondria of rat pheochromocytoma cell line PC12]. Because TiO2 has an inducible charge-separation that occurs following exposure to electromagnetic radiation of energies greater than 3.2eV, novel properties of the combined organic (DNA) and inorganic (TiO2) of these nanoparticles can be exploited.

Contact Address S. Kimura (PHS 3726) JASRI/SPring-8

Last modified 2006-04-08 17:01