大型放射光施設 SPring-8

日　時：2008年12月15日(月)　 15:00-16:30
Date : 15:00-16:30 December 15, 2008

場　所：普及棟中講堂

講演者：松井勉
Speaker : Tsutomu Matsui

Affiliate : The Scripps Research Institute

演　題：
時間分解超低温電子顕微鏡法により明らかになったT=4ウイルスの成熟化過程
Title :
Time-resolved electron cryo-microscopy revealed maturation dynamics of a pseudo T=4 viral capsid

Abstract :
Nudaurelia Capensis omega Virus (NωV) is a member of Tetravirus, T=4 non-enveloped icosahedral ssRNA viruses. The virus like particles (VLPs) of NωV coat protein were expressed and purified at neutral pH as procapsid particles (Diameter = 480 Å).  Procapsids transit to capsids (420 Å) with auto-proteolysis when the pH is lowered to < 5.2.
In present studies, the wild-type VLPs were immediately frozen at different time points after lowering pH from 7.6 to 5.0. Electron cryo-microscopy and single-particle reconstruction techniques were than used to solve the particle structures at the various time points. Difference density maps were computed and were interpreted with the crystal structure and biological data. The results reveal the sequence in which cleavage site formation occurs and illustrates the process of pseudo T=4 icosahedron formation in NωV.

担当者：熊坂（PHS：3475）
Organizer : KUMASAKA (PHS : 3475)