In vivo measurement of cerebral blood vessel response in rats
Inquiry number
SOL-0000001732
Beamline
BL28B2 (White Beam X-ray Diffraction)
Scientific keywords
| A. Sample category | biology, medicine |
|---|---|
| B. Sample category (detail) | biology (in vivo) |
| C. Technique | absorption and its secondary process |
| D. Technique (detail) | |
| E. Particular condition | 2D imaging, time-resolved (ms) |
| F. Photon energy | X-ray (4-40 keV) |
| G. Target information |
Industrial keywords
| level 1---Application area | Pharmaceuticals |
|---|---|
| level 2---Target | drug design |
| level 3---Target (detail) | drug, organism |
| level 4---Obtainable information | molphology |
| level 5---Technique | imaging |
Classification
A80.90 others
Body text
This study was focused on the radiographical evaluation of changes in small cerebral arteries that have never been previously observed. Small arteries arising directly from major trunk vessels are known as perforators that supply arterial blood to the basal nucleus. These perforators are more important than pial arteries when considering the affects of systemic disease on cerebral vessels. The distension of trunk arteries and their small branches was measured under hypercapnia and hypotension using microangiography. With this technique, various sizes of vessels were observed. In addition, pharmacological manipulations were applied to the local brain environment.
Source of the figure
No figure
Technique
The imaging system was composed of an X-ray direct-conversion-type detector incorporating an X-ray SATICON pickup tube and a high-speed X-ray shutter. Microangiographic images were obtained at a maximum rate of 30 images/s and stored in a frame memory system with a 1024×1024-pixel, 10-bit. Sequential images were obtained with an input field of view of 9.5×9.5 mm with resolution to 10 µm. The imaging field can include not only intracranial cerebral vessels but also the pterygopalatine artery which is running through the extracranial region.
Source of the figure
Presentation material for Beamline Report
Required time for experimental setup
3 hour(s)
Instruments
| Instrument | Purpose | Performance |
|---|---|---|
| Angiograpy system | Microangiography | 6-10 micron resolution |
References
Related experimental techniques
no
Questionnaire
The measurement was possible only in SPring-8. Impossible or very difficult in other facilities.
Ease of measurement
Middle
Ease of analysis
Middle
How many shifts were needed for taking whole data in the figure?
Two-three shifts