Inquiry number

INS-0000000328

ABSTRACT

  BL41XU is a high-flux biological macromolecular crystallography beamline. Experimental environment has been developed so that it can deal with the following targets by taking advantage of the brilliant undulator beam.:

1. Data collection from small (∼10 μm) crystals
2. Data collection from large unit cell (∼600 Å) crystalsii.
3. Ultra-high resolution (<0.8Å) data collection using high energy X-rays (>20 keV)
4. MAD (Multi-wavelength anomalous dispersion) or SAD (Single wavelength anomalous dispersion) by making use of the wide X-ray energy range, 6.5 ∼ 35 KeV

  In the experimental station, a diffractometer equipped with air-bearing goniometer is installed. Two types of detector, the X-ray CCD detector (Rayonix MX225HE) and the imaging plate detector (RIGAKU R-AXIS V), can be used for diffraction data collection. Installation of cryo-cooler, CRYOCOOL-G2b-LT2 (CRYO INDUSTRIES), enables sample cooling by using either nitrogen gas or helium gas. Automatic sample mounting using sample changer SPACE is available, which enable efficient sample screening.

AREA OF RESEARCH

• Structural biology
• Macromolecular crystallography
• Ultra-high resolution structure analysis
• Micro-crystallography

KEYWORDS

• Scientific field
  X-ray Protein Crystallography, Heavy-atom Isomorphous replacement, Multi-wavelength Anomalous Dispersion (MAD), Single wavelength Anomalous Dispersion (SAD), Molecular Replacement
• Equipment
  High-efficiency and Hi-speed X-ray CCD detector, Large imaging plate detector, Peltier-cooled Si-PIN photodiode, Cryo-cooler, Robotic Sample Handling System

SOURCE AND OPTICS

  This beamline employs the SPring-8 standard undulator as a light source, and a Si double crystal monochromator equipped with liquid nitrogen cooling is used to monochromatize undulator beam. The available energy range is 6.5 ∼ 17.5 keV when the first harmonics of undulator is used, and 19 ∼ 35 keV in the case of the third harmonics. The KB mirrors optics is used for beam focusing and for elimination of the higher harmonics of the undulator beam. This optics provides extremely high intensity X-rays with a size of 80 x 40μm² at the sample position. The X-ray is further collimated by a pin hole just placed before the sample, and beam size of 10, 30 and 50 μm is currently available.

Schematic View of Beamline

• X-rays at Sample

   

  Energy (wavelength) range	6.5 ∼ 17.5 keV (1.9 ∼ 0.71 Å) optional range*1: 19 ∼ 35 keV (0.65 ∼ 0.35 Å)
  Energy resolution	ΔE/E < 2 × 10-4
  Photon flux at sample position	> 1013 (without slits) 2 × 1012 (f=30mm, 1 Å)
  Beam divergence	0.1 mrad
  Beam size at sample position	80 (H) × 40 (V) mm2 f=10mm, 30mm, 50mm

   

  *1: It is necessary to change the setup of the beamline to use high energy of 19 keV or higher (0.65 Å or shorter). If you want to use such energy, please consult with the contact person before submitting your proposal.

EXPERIMENTAL STATIONS

• Protein crystal diffractometer

Detailed Information

• Public Bioxtal Beamlines HP

PUBLICATION SEARCH

* Sorry, Some parts of results are displayed using Japanese characters.

CONTACT INFORMATION

Please note that each e-mail address is followed by "@spring8.or.jp."

Kazuya HASEGAWA
SPring-8 / JASRI
1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5198
Phone : +81-(0)791-58-0833
Fax : +81-(0)791-58-0830
e-mail : kazuya

Hideo OKUMURA
SPring-8 / JASRI
1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5198
Phone : +81-(0)791-58-0833
Fax : +81-(0)791-58-0830
e-mail : okumurah