SPring-8, the large synchrotron radiation facility

Skip to content
» JAPANESE
Personal tools
 

BL41XU OUTLINE

Inquiry number

INS-0000000328

ABSTRACT

  BL41XU is a high-flux biological macromolecular crystallography beamline. This beamline has been improved for the following samples/measurements to take advantage of the highly briliant characteristics of the beam.:

  1. micro-size (~10 μm) crystals or crystals with quite a large unit cells (~600 Å)
  2. MAD (Multiwavelength anomalous dispersion) or SAD (Singlewavelength anomalous dispersion) phasing method with various energy X-rays from 6.5 ~ 35 KeV
  3. Ultra-high resolution Protein crystallography with high energy X-rays (>20 keV)

  The experimental station is equipped with a diffractometer with horizontal-axis air-bearing goniometer and detectors. Two types of cryostream coolers, nitrogen gas and helium gas, are installed on the diffractometer for experiment under cryogenic condition. The X-ray CCD detector (Rayonix MX225HE) and imaging plate detector (RIGAKU R-AXIS V) are installed and available. SPring-8 Precise Automatic Cryo-sample Exchanger (SPACE) is also installed and available.

AREA OF RESEARCH

  • Structural biology
  • Macromolecular crystallography
  • Ultra-high resolution structure analysis
  • Microcrystallography

KEYWORDS

  • Scientific field
    X-ray Protein Crystallography, Heavy-atom Isomorphous replacement, Multiwavelength Anomalous Dispersion (MAD), Singlewavelength Anomalous Dispersion (SAD), Molecular Replacement
  • Equipment
    Large imaging plate detector, High-efficiency and Hi-speed X-ray CCD detector, Peltier-cooled Si-PIN photodiode, He-cryostream cooler, Robotic Sample Handling System

SOURCE AND OPTICS

  This beamline employs the standard SPring-8 undulator as the light source, the rotated-inclined double-crystal monochromator as the monochromatizing device for the heat load removal function of the undulator beam. The rhodium-coated mirrors (horizontal and vertical) are used to focus and eliminate the higher harmonics of the undulator beam. This combination, provides the X-rays of extremely high intensity focused to a 30 μm order at the sample position in the 6.5 ∼ 17.5 keV (1.90 ∼ 0.71 Å) energy range. The energy resolution is in the order of 10-4.

Schematic View of Beamline

  • X-rays at Sample

     

    Energy (wavelength) range 6.5 ∼ 17.5 keV (1.90 ∼ 0.71 Å)
    optional range*1: 19 ∼ 35 keV (0.65 ∼ 0.35 Å)
    Energy resolution ΔE/E < 2 × 10-4
    Photon flux at sample position > 1013 (without slits)
    3 × 1012 (30 × 30 μm2, 1 Å)
    Beam divergence 0.1 mrad
    Beam size at sample position 90 (H) × 50 (V) μm2 (without slits)
    30 × 30 μm2 (slit-cut minimum)

     

    *1: It is necessary to change the setup of the beamline to use high energy of 19 keV or higher (0.65 Å or shorter). If you want to use such energy, please consult with the contact person before submitting your proposal.

EXPERIMENTAL STATIONS

Detailed Information

PUBLICATION SEARCH

* Sorry, Some parts of results are displayed using Japanese characters.

 

CONTACT INFORMATION

Please note that each e-mail address is followed by "@spring8.or.jp."

Kazuya HASEGAWA
SPring-8 / JASRI
1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5198
Phone : +81-(0)791-58-0833
Fax : +81-(0)791-58-0830
e-mail : kazuya

Hideo OKUMURA
SPring-8 / JASRI
1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5198
Phone : +81-(0)791-58-0833
Fax : +81-(0)791-58-0830
e-mail : okumurah

Last modified 2012-05-01 13:26