X-ray diffraction experiment using ultra-thin crystal
Inquiry number
SOL-0000001077
Beamline
BL41XU (Macromolecular Crystallography I)
Scientific keywords
| A. Sample category | biology, medicine, research on method, instrumentation |
|---|---|
| B. Sample category (detail) | biomolecule, crystal, protein, pharmaceuticals |
| C. Technique | X-ray diffraction |
| D. Technique (detail) | single crystal |
| E. Particular condition | low-T (~ liquid N2) |
| F. Photon energy | X-ray (4-40 keV) |
| G. Target information | molecular structure, structure analysis, function and structure |
Industrial keywords
| level 1---Application area | Pharmaceuticals |
|---|---|
| level 2---Target | process analytical technology (PAT) |
| level 3---Target (detail) | protein, drug |
| level 4---Obtainable information | crystal structure |
| level 5---Technique | diffraction |
Classification
A80.50 Pharmaceuticals, M10.10 single crystal diffraction
Body text
Fragellin is the protein that is one of the component of bacterial fragellar. The F41, fragment of the fragellin, was crystallized only in the thickness less than 10µm (Fig. 1).
Figure 1. Picture of ultra-thin crystal of fragellin fragment F41
scale bar indicates 300m.
[ F. A. Samatey, K. Imada, F. Vonderviszt, Y. Shirakihara and K. Namba, Journal of Structural Biology 12, 106-111 (2000), Fig. 1,
©2000 Elsevier B. V. ]
Using neither X-ray generator in laboratory nor bending magnet beamline in past synchrotron facilities, it was very difficult to obtain X-ray diffraction data from such an ultra-thin crystal with sufficient quality. High brilliant X-ray beam from undulator of BL41XU enable to measure X-ray diffraction data in high resolution and with sufficient accuracy.
Source of the figure
Original paper/Journal article
Journal title
F. A. Samatey, et al., J Struct Biol., 132(2), 106-111 Nov (2000)
Figure No.
1
Technique
Source of the figure
No figure
Required time for experimental setup
0 hour(s)
Instruments
| Instrument | Purpose | Performance |
|---|---|---|
| Protein Crystal Diffractometer | To record diffraction data |
References
| Document name |
|---|
| F. A. Samatey, et al., J Struct Biol., 132(2), 106-111 Nov. (2000) |
Related experimental techniques
Questionnaire
The measurement was possible only in SPring-8. Impossible or very difficult in other facilities.
This solution is an application of a main instrument of the beamline.
Ease of measurement
Middle
Ease of analysis
Middle
How many shifts were needed for taking whole data in the figure?
Four-nine shifts