Stepwise assembly of the catalytic core of [NiFe]-hydrogenase（Press Release）
- Release Date
- 08 Feb, 2023
- BL09XU
8 Feb, 2023
Japan Synchrotron Radiation Research Institute
Technische Universität Berlin
Humboldt-Universität zu Berlin
Max Planck Institute
Deutsches Elektronen Synchrotron
An international research team, involving members from the TU Berlin, HU Berlin, MPI Mülheim, JASRI (Hyōgo, Japan) and DESY (Hamburg) deciphered the multistep incorporation process of the catalytic NiFe(CN)2(CO) cofactor into [NiFe]-hydrogenase by isolating previously uncharacterized protein intermediates trapped in different stages of maturation. These were analyzed using biochemical and a broad range of spectroscopic techniques. 【Publication】 |
Hydrogenases hold a promising future for hydrogen-based technologies and as a blueprint for chemically synthesized catalysts. The results of the study provide detailed insights into the assembly process of the intricate catalytic core of [NiFe]-hydrogenase. Moreover, the isolated intermediates serve as an ideal platform to study (semi-)artificial [NiFe]-hydrogenases equipped with synthetic iron complexes and Ni substitutes, potentially generating "chemzymes" with alternative catalytic functions.
The research results were published in Nature Chemical Biology on January 26, 2023.
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