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Stepwise assembly of the catalytic core of [NiFe]-hydrogenase(Press Release)

Release Date
08 Feb, 2023
  • BL09XU

8 Feb, 2023
Japan Synchrotron Radiation Research Institute
Technische Universität Berlin
Humboldt-Universität zu Berlin
Max Planck Institute
Deutsches Elektronen Synchrotron

An international research team, involving members from the TU Berlin, HU Berlin, MPI Mülheim, JASRI (Hyōgo, Japan) and DESY (Hamburg) deciphered the multistep incorporation process of the catalytic NiFe(CN)2(CO) cofactor into [NiFe]-hydrogenase by isolating previously uncharacterized protein intermediates trapped in different stages of maturation. These were analyzed using biochemical and a broad range of spectroscopic techniques.

Title: Stepwise assembly of the active site of [NiFe]-hydrogenase
Authors: Giorgio Caserta, Sven Hartmann, Casey Van Stappen, Chara Karafoulidi-Retsou, Christian Lorent, Stefan Yelin, Matthias Keck, Janna Schoknecht, Ilya Sergueev, Yoshitaka Yoda, Peter Hildebrandt, Christian Limberg, Serena DeBeer, Ingo Zebger, Stefan Frielingsdorf and Oliver Lenz
Journal: Nature Chemical Biology
DOI: https://doi.org/10.1038/s41589-022-01226-w


 Hydrogenases hold a promising future for hydrogen-based technologies and as a blueprint for chemically synthesized catalysts. The results of the study provide detailed insights into the assembly process of the intricate catalytic core of [NiFe]-hydrogenase. Moreover, the isolated intermediates serve as an ideal platform to study (semi-)artificial [NiFe]-hydrogenases equipped with synthetic iron complexes and Ni substitutes, potentially generating "chemzymes" with alternative catalytic functions.
 The research results were published in Nature Chemical Biology on January 26, 2023.


Yoshitaka Yoda,
Japan Synchrotron Radiation Research Institute (JASRI)
Precision Spectroscopy Division
 TEL:+81-791-58-0802 (Ext:3939)

User Administration Div. Information and Outreach Sec.,
Japan Synchrotron Radiation Research Institute (JASRI)
 TEL: +81-791-58-2785

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