SPring-8, the large synchrotron radiation facility

Speaker : Prof. John E. Johnson

Language : ENGLISH

Affiliate : Department of Molecular Biology, The Scripps Research Institute

Title : Maturation of dsDNA Bacteriophage: insights into elegantly programmed nanomachines

Abstract :
The accessibility of bacteriophage maturation to structural, biophysical and genetic methods has enhanced our understanding of novel processes in biological dynamics. These include identifying mechanisms that create energy landscapes for exothermic virus maturation as well as discovering a means of directing the effects of thermal fluctuations for particle development through a Brownian ratchet. The structurally encoded program that directs particle maturation will be described.

References:
(1)Tang, J., Lander, G. C., Olia, A., Li, R., Casjens, S., Prevelige, P., Jr., Cingolani, G., Baker, T. S., and Johnson, J. E. 2011. Peering down the barrel of a bacteriophage portal: the genome packaging and release valve in p22. Structure 19:496-502.

(2)Gertsman, I., Gan, L., Guttman, M., Lee, K., Speir, J. A., Duda, R. L., Hendrix, R. W., Komives, E. A., and Johnson, J. E. 2009. An unexpected twist in viral capsid maturation. Nature 458:646-651.

(3)Johnson, J. E. 2010. Virus particle maturation: insights into elegantly programmed nanomachines. Curr Opin Struct Biol 20:210-6.

(4)Lander, G. C., Baudoux, A. C., Azam, F., Potter, C. S., Carragher, B., and Johnson, J. E. 2012. Capsomer dynamics and stabilization in particles with T=12 quasi-symmetry; the cryoEM reconstructions of the marine bacteriophage SIO-2 and its procapsid. Structure accepted for publication.

Organizer : Kazuya Hasegawa
Mail : kazuya@spring8.or.jp
PHS : 3595