Nuclear Resonance Vibrational Spectroscopy of the nitrogenase
Inquiry number
SOL-0000000924
Beamline
BL09XU (HAXPES I)
Scientific keywords
| A. Sample category | organic material |
|---|---|
| B. Sample category (detail) | protein, environmental material |
| C. Technique | inelastic scattering, nuclear excitation, Mössbauer effect |
| D. Technique (detail) | nuclear excitation, nuclear resonance |
| E. Particular condition | low-T (~ liquid N2), time-resolved (ns) |
| F. Photon energy | X-ray (4-40 keV) |
| G. Target information | local structure, phonon |
Industrial keywords
| level 1---Application area | environment, Pharmaceuticals |
|---|---|
| level 2---Target | catalysis, drug design |
| level 3---Target (detail) | protein |
| level 4---Obtainable information | local structure |
| level 5---Technique | scattering |
Classification
A80.34 catalysis, A80.40 environmental materials
Body text
Nuclear Resonance Vibrational Spectroscopy (NRVS) is a unique technique to study element-specific phonon density of states. Using this technique, one can measure the element-specific vibrational states of the condensed matter, the amorphous and the liquid containing Mössbauer nuclei. The figure shows the phonon density of states of the nitrogenase. These data reveal the fact that how each vibrational mode contribute to the whole system of the enzyme.
(a) NRVS spectrum for the nitrogenase enzyme. (b) the twisting normal mode for the FeMo-cofactor cluster, predicted to occur around 10 meV in the NRVS spectrum. The cluster was simplified by making all Mo (orange) ligands oxygen atoms (red). Fe atoms are purple and Sulfurs are yellow.
Source of the figure
Bulletin from SPring-8
Bulletin title
Research Frontiers 2003
Page
69
Technique
Source of the figure
Required time for experimental setup
Instruments
References
Related experimental techniques
Questionnaire
The measurement was possible only in SPring-8. Impossible or very difficult in other facilities.
This solution is an application of a main instrument of the beamline.
Similar experiments account for more than 30% of the beamline's subject.
Ease of measurement
Middle
Ease of analysis
Middle
How many shifts were needed for taking whole data in the figure?
Two-three shifts